Marie Soukupová

Solid experience in standard experimental models of epilepsy (pilocarpine induced status epilepticus, kainate model, kindling) and models of pain (acetic acid writhing test, plantar test, von Frey test, formalin test, tail-flick test) in rodents; behavioral testing (rota rod, activity cage) in rats.

Stereotaxic surgery in rodent’s experience.

Experience in electrophysiology (one cell recording; software Spike2) and EEG monitoring (software AcqKnowledge) in rats.

Experience in high performance liquid chromatography (HPLC) and separation of biological samples (solid phase extraction, liquid-liquid extraction)

Good computer skills (standard Microsoft Office software applications;   statistical analysis software – GrafPad, SigmaStat; chromatographic software CLASS-VP; CSW; LC Solution; Galaxie WorkStation)

The 9 month period of time research stay at the Department of Neuropharmacology, Institute of Pharmacology and Toxicology, Faculty of Medicine and Surgery, Second University of Naples, Italy; under the supervision of Prof. S. Maione focusing on the role of the hind brain regions (periaqueductal gray matter and rostroventral medulla) in pain control using the classic pain tests, the in vivo microdialysis and HPLC techniques.

1: Soukupová M, Binaschi A, Falcicchia C, Zucchini S, Roncon P, Palma E, Magri E, Grandi E, Simonato M. Impairment of GABA release in the hippocampus at the time of the first spontaneous seizure in the pilocarpine model of temporal lobe epilepsy. Exp Neurol. 2014 Jul;257:39-49.

 2: Buzzi A, Chikhladze M, Falcicchia C, Paradiso B, Lanza G, Soukupova M, Marti M, Morari M, Franceschetti S, Simonato M. Loss of cortical GABA terminals in Unverricht-Lundborg disease. Neurobiol Dis. 2012 Aug;47(2):216-24.

 3: Palazzo E, Marabese I, Soukupova M, Luongo L, Boccella S, Giordano C, de Novellis V, Rossi F, Maione S. Metabotropic glutamate receptor subtype 8 in the amygdala modulates thermal threshold, neurotransmitter release, and rostral ventromedial medulla cell activity in inflammatory pain. J Neurosci. 2011 Mar 23;31(12):4687-97.

 4: Giordano C, Siniscalco D, Melisi D, Luongo L, Curcio A, Soukupova M, Palazzo E, Marabese I, De Chiaro M, Rimoli MG, Rossi F, Maione S, de Novellis V. The galactosylation of N(ω)-nitro-L-arginine enhances its anti-nocifensive or anti-allodynic effects by targeting glia in healthy and neuropathic mice. Eur J Pharmacol. 2011 Apr 10;656(1-3):52-62.

 5: Soukupová M, Palazzo E, De Chiaro M, Gatta L, Migliozzi AL, Guida F, Luongo L, Giordano C, Siniscalco D, De Novellis V, Marabese I, Krsiak M, Maione S. Effects of URB597, an inhibitor of fatty acid amide hydrolase (FAAH), on analgesic activity of paracetamol. Neuro Endocrinol Lett. 2010;31(4):507-11

 6: Palazzo E, Guida F, Migliozzi A, Gatta L, Marabese I, Luongo L, Rossi C, de Novellis V, Fernández-Sánchez E, Soukupova M, Zafra F, Maione S. Intraperiaqueductal gray glycine and D-serine exert dual effects on rostral ventromedial medulla ON- and OFF-cell activity and thermoceptive threshold in the rat. J Neurophysiol. 2009 Dec;102(6):3169-79.

 7: Tůma P, Soukupová M, Samcová E, Stulík K. A determination of submicromolar concentrations of glycine in periaqueductal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection. Electrophoresis. 2009 Oct;30(19):3436-41.

 8: Soukupova M, Dolezal T, Krsiak M. Synergistic interaction between rilmenidine and ibuprofen in the writhing test in mice. Neuro Endocrinol Lett. 2009;30(2):215-20.

 9: Soukupová M, Dolezal T, Krsiak M. The synergistic interaction between rilmenidine and paracetamol in the writhing test in mice. Naunyn Schmiedebergs Arch Pharmacol. 2009 Jun;379(6):575-80.

 10: Prochazkova M, Zanvit P, Soukupova M, Horakova L, Dolezal T, Prokesova L, Kršiak M. Expression and production of spinal cyclooxygenase 1 and cyclooxygenase 2 in the spared nerve injury model of neuropathic pain. Eur J Pain. 2009 Sept 13 (S1):S74a-S74.

 11: Nobilis M, Vybíralová Z, Krízová V, Kubícek V, Soukupová M, Lamka J, Szotáková B, Skálová L. Sensitive chiral high-performance liquid chromatographic determination of anthelmintic flubendazole and its phase I metabolites in blood plasma using UV photodiode-array and fluorescence detection Application to pharmacokinetic studies in sheep. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Dec 1;876(1):89-96.

Main research interests and intentions:  Basic research in Epilepsy;  Pharmacology of Pain;  Determination of concentrations of neurotransmitters playing role in epilepsy and pain processing in microdialysis perfusates from rat brains via HPLC.